Meeting Abstracts
» Discovery of MIF and Cyclophilin A as Potential Molecular Targets in Non-small Cell Lung Cancer from Protein Expression Profiles

Campa M, Wang M, Howard, B., Fitzgerald M, Patz, Jr. EF
Departments of Radiology and Chemistry, Duke University Durham, NC 27710

Abstract

Current diagnostic and therapeutic strategies for lung cancer have had no significant impact on lung cancer mortality over the last several decades. This study used a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) discovery platform to generate protein expression profiles in search of overexpressed proteins in lung tumors as potentially novel molecular targets. Two differentially expressed protein peaks at m/z 12,338 and 17,882 in the MALDI-TOF spectra were identified in lung tumor specimens as macrophage migration inhibitory factor (MIF) and cyclophilin A (CyP-A), respectively. Overexpression of both proteins was confirmed by Western blotting, and CyP-A was localized to the tumor cells by immunohistochemistry. These data demonstrate the feasibility of using a MALDI-TOF platform to generate protein expression profiles and to identify potential molecular targets for cancer diagnostics and therapeutics.

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